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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
09/11/2017 |
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Actualizado : |
12/12/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Soil and rhizosphere bacterial diversity in maize agro-ecosystem. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 35-51. |
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ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
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DOI : |
10.5539/sar.v6n3p |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. |
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Contenido : |
ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial ... Presentar Todo |
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Palabras claves : |
DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668
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Marc : |
LEADER 02541naa a2200289 a 4500
001 1057747
005 2018-12-12
008 2017 bl uuuu u00u1 u #d
022 $a1927-050X (Print) // 1927-0518 (Online)
024 7 $a10.5539/sar.v6n3p$2DOI
100 1 $aFEDERICI, M.
245 $aSoil and rhizosphere bacterial diversity in maize agro-ecosystem.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017.
520 $aABSTRACT. Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices. Copyright © Canadian Center of Science and Education
653 $aDGGE
653 $aMETAGENOMICS
653 $aPYROSEQUENCING
653 $aRHIZOSPHERE
653 $aSOIL BACTERIA
653 $aTRANSGENIC MAIZE
700 1 $aBAJSA, N.
700 1 $aLAGURARA, P.
700 1 $aREVALE, S.
700 1 $aMARCONDES, J.A.
700 1 $aDALLA RIZZA, M.
773 $tSustainable Agriculture Research, 2017$gv.6 (3): 35-51.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/08/2016 |
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Actualizado : |
28/02/2020 |
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Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
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Autor : |
BLUMETTO, O.; CASTAGNA, A.; CARDOZO, G.; RUGGIA, A.; SCARLATO, S.; TISCORNIA, G.; GARCIA, F.; AGUERRE, V. |
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Afiliación : |
OSCAR RICARDO BLUMETTO VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRES CASTAGNA DU PRE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA PAOLA RUGGIA CHIESA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANTIAGO SCARLATO GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUADALUPE TISCORNIA TOSAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FELIPE LUIS GARCIA OLASO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VERONICA AGUERRE ANTIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Ecosystem Integrity Index: A New Tool for Ecosystem Services Evaluation in Livestock Production Systems. |
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Complemento del título : |
Ecological Goods & Services of Rangelands and Pasturelands. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
In: PROCEEDINGS OF THE INTERNATIONAL RANGELAND CONGRESS, 10th, "The Future Management of Grazing and Wild Lands in a High-Tech World". Saskatoon, SK, Canadá, July 16-22, 2016, p. 448-449. |
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Idioma : |
Inglés |
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Contenido : |
INTRODUCTION.
Animal production system based on rangeland are very complex being its management closely related to the functioning of the ecosystem in which it is supoorted. For assessing the environmental impact of livestock management many variables need to be considered, making difficult and expensive to obtain a comprehensive overview of the ecosystem state and evolution. Therefore a qualitative and quantitative assessment tool was developed in order to evaluate the integrity of the ecosystem under productive use that may be applied in a simple and practical process. The aim of this study was to evaluate the performance of the index in real production systems that were part of a participatory research project. |
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Palabras claves : |
ANIMAL PRODUCTION; BIODIVERSITY; GRASSLAND; LIVESTOCK; RANGELAND; SUSTAINABLE PRODUCTION. |
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Thesagro : |
BIODIVERSIDAD; GANADO DE CARNE; PASTIZALES; PASTURAS; PRODUCCIÓN ANIMAL; SUSTENTABILIDAD PRODUCTIVA. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5937/1/Blumetto-O.-2016.-Proc.10th-Rang.Cong..pdf
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Marc : |
LEADER 01816nam a2200337 a 4500
001 1055328
005 2020-02-28
008 2016 bl uuuu u01u1 u #d
100 1 $aBLUMETTO, O.
245 $aEcosystem Integrity Index$bA New Tool for Ecosystem Services Evaluation in Livestock Production Systems.$h[electronic resource]
260 $aIn: PROCEEDINGS OF THE INTERNATIONAL RANGELAND CONGRESS, 10th, "The Future Management of Grazing and Wild Lands in a High-Tech World". Saskatoon, SK, Canadá, July 16-22, 2016, p. 448-449.$c2016
520 $aINTRODUCTION. Animal production system based on rangeland are very complex being its management closely related to the functioning of the ecosystem in which it is supoorted. For assessing the environmental impact of livestock management many variables need to be considered, making difficult and expensive to obtain a comprehensive overview of the ecosystem state and evolution. Therefore a qualitative and quantitative assessment tool was developed in order to evaluate the integrity of the ecosystem under productive use that may be applied in a simple and practical process. The aim of this study was to evaluate the performance of the index in real production systems that were part of a participatory research project.
650 $aBIODIVERSIDAD
650 $aGANADO DE CARNE
650 $aPASTIZALES
650 $aPASTURAS
650 $aPRODUCCIÓN ANIMAL
650 $aSUSTENTABILIDAD PRODUCTIVA
653 $aANIMAL PRODUCTION
653 $aBIODIVERSITY
653 $aGRASSLAND
653 $aLIVESTOCK
653 $aRANGELAND
653 $aSUSTAINABLE PRODUCTION
700 1 $aCASTAGNA, A.
700 1 $aCARDOZO, G.
700 1 $aRUGGIA, A.
700 1 $aSCARLATO, S.
700 1 $aTISCORNIA, G.
700 1 $aGARCIA, F.
700 1 $aAGUERRE, V.
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